Human need interaction and communication between organs in the whole body, such as the "dialogue" between tumor organoid and immune system, and the co-culture of tumor organoid and live organoid considering metabolism.
The traditional culture method lacks the simulation of biomechanical signals (fluid flow, matrix hardness, tensile deformation, etc.) in vivo, which leads to further improvement of the functional indicators of organoids and the correlation between tumor in situ and clinical.
The traditional organoid culture and screening platform is a 24, 96 or 384 well plate. The success rate of organoid culture is low, the cycle is long, the stability and reproducibility are poor, and the cost of cells and reagents is large. The differences in size, structure, function and gene expression of the same batch of organoids lead to great variability in the results of subsequent biological experiments and drug sensitivity tests.
Organoids-on-chips technology can complement the advantages of Organoid and Organ chip technology, and Organoids technology can improve the bionics of cell source in Organ chip technology. Daxiang biotech makes full use of the advantages of the two technologies to form an Organois chip platform with the following advantages and characteristics:
Through ingenious fluid channel design, solve the problem of nutrient metabolism disorder in traditional static culture mode and the simulation of bio-mechanical signals, improve the success rate of organoid culture, and achieve high-quality cultivation of larger and larger organoids for a long time.
Through the miniaturization of the chip, the cost of similar organs and consumables can be saved. More importantly, the secretion factors in the micro-culture system are easy to accumulate to a higher concentration and play their functions, thus realizing the cultivation of a small number of samples, which is expected to reduce the cultivation cycle and improve efficiency.
The chip uses non-PDMS materials to reduce the non-specific adsorption of small molecule drugs.
Through the quality control of the chip and the optimization of the experimental conditions, the stability and reproducibility of the product are improved, and the differences between the size, structure, function and gene expression of the same batch of organs are reduced.
The unique chip design improves the usability of the chip and reduces the difficulty of operation and the result variation introduced by human operation as much as possible.
Realize a one-stop chip solution from organoid culture to high-flux drug sensitivity test.
[1] Method of the Year 2017: Organoids. Nat. Methods, 2018, 15, 1.
[2] Modeling development and disease with organoids. Cell, 2016, 165, 1586-1597.
[3] Engineering human islet organoids from iPSCs using an organ-on-chip platform. Lab Chip, 2019,19, 948-958.
[4] Organoid-on-a-chip and body-on-a-chip systems for drug screening and disease modeling. Drug Discov. Today. 2016,21,1399-1411.-a-chip systems for drug screening and disease modeling. Drug Discov. Today. 2016,21,1399-1411.
